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ATCC human intestinal epithelial cells
Human Intestinal Epithelial Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 15198 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+intestinal+epithelial+caco+2+cells/Caco-2/pm42171256-57-0-5
Average 99 stars, based on 15198 article reviews

human intestinal epithelial cells - by Bioz Stars, 2026-09

99/100 stars

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Article Title: Albumin coating of magnetic nanoparticles for imaging, tracking and delivery through biological barriers.
Article Snippet: Human intestinal epithelial Caco-2 cells (ATCC n. HTB-37TM) were grown at 37 °C, under a humidified

Cell Culture:

Article Title: Oxidized Albumin Induces Renal Tubular Cell Death and Promotes the Progression of Renal Diseases Through Ferroptosis
Article Snippet: L-AA, maleimide, and other chemicals were sourced from Sigma-Aldrich (St. Louis, MO, USA). .. Rat renal tubular epithelial NRK-52E and human intestinal epithelial Caco-2 cells (ATCC, Rockville, MD, USA) were cultured in Dulbecco’s Modified Eagle Medium (DMEM)/F12 supplemented with 5% Fetal Bovine Serum (FBS) and 1% antibiotic– antimycotic solution under a humidified atmosphere of 5% CO2/95% air at 37 ◦C. ..

Article Title: Oxidized Albumin Induces Renal Tubular Cell Death and Promotes the Progression of Renal Diseases Through Ferroptosis
Article Snippet: L-AA, maleimide, and other chemicals were sourced from Sigma-Aldrich (St. Louis, MO, USA). .. Rat renal tubular epithelial NRK-52E and human intestinal epithelial Caco-2 cells (ATCC, Rockville, MD, USA) were cultured in Dulbecco’s Modified Eagle Medium (DMEM)/F12 supplemented with 5% Fetal Bovine Serum (FBS) and 1% antibiotic–antimycotic solution under a humidified atmosphere of 5% CO 2 /95% air at 37 °C. ..

Modification:

Article Title: Oxidized Albumin Induces Renal Tubular Cell Death and Promotes the Progression of Renal Diseases Through Ferroptosis
Article Snippet: L-AA, maleimide, and other chemicals were sourced from Sigma-Aldrich (St. Louis, MO, USA). .. Rat renal tubular epithelial NRK-52E and human intestinal epithelial Caco-2 cells (ATCC, Rockville, MD, USA) were cultured in Dulbecco’s Modified Eagle Medium (DMEM)/F12 supplemented with 5% Fetal Bovine Serum (FBS) and 1% antibiotic–antimycotic solution under a humidified atmosphere of 5% CO 2 /95% air at 37 °C. ..

Membrane:

Article Title: Human origin probiotic lactobacillus rhamnosus HL-200 to reduce leaky gut by metabolizing ethanolamine
Article Snippet: .. Human intestinal epithelial Caco-2 cells (American Type Culture Collection) were seeded on apical chamber made of polyester membrane filters with 0.4 μm pore size of 12-well transwell plates (Costar) at a density of 3×105/well. ..

Pore Size:

In Vitro:

Article Title: A Combination of Plant-Derived Extracts Modulates Nutrient-Responsive Metabolic Signalling in an In Vitro Gut–Liver–Adipose Model
Article Snippet: .. Human intestinal epithelial Caco-2 cells and enteroendocrine NCI-H716 cells (American Type Culture Collection, ATCC ® , Manassas, VA, USA) were used to create an in vitro intestinal model that mimics the epithelial–enteroendocrine interface, allowing simultaneous evaluation of epithelial transport and GLP-1 secretion. .. Caco-2 cells were cultured in Advanced Dulbecco’s Modified Eagle Medium (Adv-DMEM; GIBCO ® , Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% FBS, 2 mM L-glutamine, and 1% penicillin–streptomycin.

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Cell viability assay of Gastrodia elata rhizome extract on intestinal cells (Caco-2). Data are expressed as mean ± SD (%) of 5 independent experiments, each performed in triplicate and normalised to the control (0%) line. p < 0.05 vs. control.

Journal: Nutrients

Article Title: A Combination of Plant-Derived Extracts Modulates Nutrient-Responsive Metabolic Signalling in an In Vitro Gut–Liver–Adipose Model

doi: 10.3390/nu18091393

Figure Lengend Snippet: Cell viability assay of Gastrodia elata rhizome extract on intestinal cells (Caco-2). Data are expressed as mean ± SD (%) of 5 independent experiments, each performed in triplicate and normalised to the control (0%) line. p < 0.05 vs. control.

Article Snippet: Human intestinal epithelial Caco-2 cells and enteroendocrine NCI-H716 cells (American Type Culture Collection, ATCC ® , Manassas, VA, USA) were used to create an in vitro intestinal model that mimics the epithelial–enteroendocrine interface, allowing simultaneous evaluation of epithelial transport and GLP-1 secretion.

Techniques: Viability Assay, Control

Effects of individual extracts on cell viability in Caco-2 cells under dose–response conditions, and effects of their combination under time-dependent conditions (1–6 h). In ( A ), dose–response effects of Paeonia lactiflora root extract; in ( B ), dose–response effects of Morus alba leaf extract; and in ( C ), comparative effects of the combination ( Gastrodia elata + Morus alba + Paeonia lactiflora ) versus individual components. Gastrodia elata concentration (0.1 mg/mL) were based on published dose–response data as described in and reported in . Data are expressed as mean ± SD (%) from 5 independent experiments, each performed in triplicate and normalized to the control (0%). * p < 0.05 vs. control.

Journal: Nutrients

Article Title: A Combination of Plant-Derived Extracts Modulates Nutrient-Responsive Metabolic Signalling in an In Vitro Gut–Liver–Adipose Model

doi: 10.3390/nu18091393

Figure Lengend Snippet: Effects of individual extracts on cell viability in Caco-2 cells under dose–response conditions, and effects of their combination under time-dependent conditions (1–6 h). In ( A ), dose–response effects of Paeonia lactiflora root extract; in ( B ), dose–response effects of Morus alba leaf extract; and in ( C ), comparative effects of the combination ( Gastrodia elata + Morus alba + Paeonia lactiflora ) versus individual components. Gastrodia elata concentration (0.1 mg/mL) were based on published dose–response data as described in and reported in . Data are expressed as mean ± SD (%) from 5 independent experiments, each performed in triplicate and normalized to the control (0%). * p < 0.05 vs. control.

Techniques: Concentration Assay, Control

Evaluation of intestinal permeability in Caco-2 cells. ( A ) TEER values measured using EVOM3 voltohmmeter over time (1–6 h). ( B – D ) TJ protein levels (Claudin-1, Occludin, and ZO-1, respectively) were quantified by ELISA after 6 h of treatment. ( E ) Absorption rate over time (1–6 h), calculated according to the equation J = Jmax [C]/(Kt + [C]) using fluorescent probe. ( F ) GLP-1 secretion was measured after 6 h by ELISA kit. The botanical extracts used in this study were derived from Morus alba leaves, Paeonia lactiflora roots, and Gastrodia elata rhizomes. Data in panel ( A ) and ( F ) are presented as mean ± SD from five independent experiments each performed in triplicate. Data in panels ( B – E ) are expressed as mean ± SD from 5 independent experiments, each performed in triplicate and normalized to the control (0%).* p < 0.05 vs. control; α p < 0.05 vs. single components.

Journal: Nutrients

Article Title: A Combination of Plant-Derived Extracts Modulates Nutrient-Responsive Metabolic Signalling in an In Vitro Gut–Liver–Adipose Model

doi: 10.3390/nu18091393

Figure Lengend Snippet: Evaluation of intestinal permeability in Caco-2 cells. ( A ) TEER values measured using EVOM3 voltohmmeter over time (1–6 h). ( B – D ) TJ protein levels (Claudin-1, Occludin, and ZO-1, respectively) were quantified by ELISA after 6 h of treatment. ( E ) Absorption rate over time (1–6 h), calculated according to the equation J = Jmax [C]/(Kt + [C]) using fluorescent probe. ( F ) GLP-1 secretion was measured after 6 h by ELISA kit. The botanical extracts used in this study were derived from Morus alba leaves, Paeonia lactiflora roots, and Gastrodia elata rhizomes. Data in panel ( A ) and ( F ) are presented as mean ± SD from five independent experiments each performed in triplicate. Data in panels ( B – E ) are expressed as mean ± SD from 5 independent experiments, each performed in triplicate and normalized to the control (0%).* p < 0.05 vs. control; α p < 0.05 vs. single components.

Techniques: Permeability, Enzyme-linked Immunosorbent Assay, Derivative Assay, Control

The percentage viability of Caco-2 cells follow 48 h exposure to AA-7 ( a ) and glucose uptake inhibition kinetics with fluorescent glucose 2-NBDG, following treatment with AA-7 ( b ). Data are presented as mean ± SD for three or more independent trials with consistent findings. The use of different letters (a-c) identifies means which exhibit significant differences ( p < 0.05).

Journal: Scientific Reports

Article Title: Bee pollen-derived peptide with dual DPP-IV Inhibition and glucose transport modulation

doi: 10.1038/s41598-026-39009-1

Figure Lengend Snippet: The percentage viability of Caco-2 cells follow 48 h exposure to AA-7 ( a ) and glucose uptake inhibition kinetics with fluorescent glucose 2-NBDG, following treatment with AA-7 ( b ). Data are presented as mean ± SD for three or more independent trials with consistent findings. The use of different letters (a-c) identifies means which exhibit significant differences ( p < 0.05).

Article Snippet: Human intestinal epithelial cell line Caco-2 cells (ATCC ® HTB-37TM) were supplied by the American Type Culture Collection (ATCC, Manassas, VA, USA), before being cultured in Eagle’s Minimum Essential Medium (EMEM) which was supplemented using L-glutamine and 10% fetal bovine serum (Gibco, Rockville, MD, USA).

Techniques: Inhibition

Cytotoxicity and inhibitory effects on RV replication of different HSP90 inhibitors. ( A ) Plots for cell viability of MA104, Caco-2, and HT-29 cells after treatment with NVP-HSP990, GA, or 17-AAG at indicated concentrations for 24 h. Cell viability was tested using the CCK-8 assay. ( B ) Plots for RV (Wa and SA11 strains) inhibition in MA104, Caco-2, and HT-29 cells after treatment with NVP-HSP990, GA, or 17-AAG at indicated concentrations for 24 h. RV replication was tested by PFA, and IC 50 values are indicated at the top of each plot. The experiments were performed in triplicate, and the data are presented as mean ± SEM and are representative of four ( A ) and two ( B ) independent experiments. ns, not significant; * P < 0.05, ** P < 0.01, **** P < 0.0001 (two-way ANOVA).

Journal: Journal of Virology

Article Title: A small-molecule HSP90 inhibitor, NVP-HSP990, alleviates rotavirus infection

doi: 10.1128/jvi.01883-25

Figure Lengend Snippet: Cytotoxicity and inhibitory effects on RV replication of different HSP90 inhibitors. ( A ) Plots for cell viability of MA104, Caco-2, and HT-29 cells after treatment with NVP-HSP990, GA, or 17-AAG at indicated concentrations for 24 h. Cell viability was tested using the CCK-8 assay. ( B ) Plots for RV (Wa and SA11 strains) inhibition in MA104, Caco-2, and HT-29 cells after treatment with NVP-HSP990, GA, or 17-AAG at indicated concentrations for 24 h. RV replication was tested by PFA, and IC 50 values are indicated at the top of each plot. The experiments were performed in triplicate, and the data are presented as mean ± SEM and are representative of four ( A ) and two ( B ) independent experiments. ns, not significant; * P < 0.05, ** P < 0.01, **** P < 0.0001 (two-way ANOVA).

Article Snippet: Human intestinal epithelial cell lines Caco-2 cells (ATCC: HTB-37) and HT-29 cells (ATCC: HTB-38) were from ATCC and kept in our institute.

Techniques: CCK-8 Assay, Inhibition

NVP-HSP990 significantly inhibited RV replication, viral gene transcription, and antigen expression. ( A ) Caco-2 cells were infected with RV (Wa or SA11 strains) at varying MOIs (0.001–10), followed by treatment with 100 nM NVP-HSP990 or an equal volume of DMSO (as a control) for 24 h. Viral replication was assessed using PFA. ( B ) Schematic diagram illustrating the timing of NVP-HSP990 addition and removal. ( C ) Caco-2 cells were infected with RV (MOI = 1) and treated with 100 nM NVP-HSP990 or with DMSO as control at indicated infection periods as shown in panel B. Viral replication was tested with PFA at 20 h p.i. ( D ) Caco-2 cells were infected with RV (MOI = 1) and treated with 100 nM HSP990 at 0, 4, 8, 12 h p.i. or treated with DMSO at 0 h p.i. Virus replication was tested with PFA at 24 h p.i. ( E ) Caco-2 cells were infected with RV (MOI = 1) and further cultivated with DMEM containing 100 nM NVP-HSP990 or DMSO as control for 18 h. Then the infected cells were harvested for qPCR analysis of the expression of RV genes encoding the VP2, VP6, NSP4, and NSP5 proteins. ( F ) Caco-2 cells were mock-infected with PBS or infected with RV (MOI = 1) and further cultivated with DMEM containing 100 nM NVP-HSP990 or DMSO as control for 18 h. Then the cells were harvested for WB analysis of RV structural proteins VP6 and VP7. ( G ) Caco-2 cells growing on coverslips were mock-infected with PBS or infected with RV (MOI = 3) and treated with 100 nM NVP-HSP990 or DMSO as control for 18 h. Then the infected cells were subjected to immunostaining of RV VP6 antigens (red). Data are presented as mean ± SEM ( A, C, D, E ). The experiments were performed in triplicate ( A, C, D, E ), and the data are representative of two independent experiments ( A, C–G ). ns, not significant; ** P < 0.01, *** P < 0.001, **** P < 0.0001 (two-way ANOVA [ A, C, E ] and one-way ANOVA [ D ]).

Journal: Journal of Virology

Article Title: A small-molecule HSP90 inhibitor, NVP-HSP990, alleviates rotavirus infection

doi: 10.1128/jvi.01883-25

Figure Lengend Snippet: NVP-HSP990 significantly inhibited RV replication, viral gene transcription, and antigen expression. ( A ) Caco-2 cells were infected with RV (Wa or SA11 strains) at varying MOIs (0.001–10), followed by treatment with 100 nM NVP-HSP990 or an equal volume of DMSO (as a control) for 24 h. Viral replication was assessed using PFA. ( B ) Schematic diagram illustrating the timing of NVP-HSP990 addition and removal. ( C ) Caco-2 cells were infected with RV (MOI = 1) and treated with 100 nM NVP-HSP990 or with DMSO as control at indicated infection periods as shown in panel B. Viral replication was tested with PFA at 20 h p.i. ( D ) Caco-2 cells were infected with RV (MOI = 1) and treated with 100 nM HSP990 at 0, 4, 8, 12 h p.i. or treated with DMSO at 0 h p.i. Virus replication was tested with PFA at 24 h p.i. ( E ) Caco-2 cells were infected with RV (MOI = 1) and further cultivated with DMEM containing 100 nM NVP-HSP990 or DMSO as control for 18 h. Then the infected cells were harvested for qPCR analysis of the expression of RV genes encoding the VP2, VP6, NSP4, and NSP5 proteins. ( F ) Caco-2 cells were mock-infected with PBS or infected with RV (MOI = 1) and further cultivated with DMEM containing 100 nM NVP-HSP990 or DMSO as control for 18 h. Then the cells were harvested for WB analysis of RV structural proteins VP6 and VP7. ( G ) Caco-2 cells growing on coverslips were mock-infected with PBS or infected with RV (MOI = 3) and treated with 100 nM NVP-HSP990 or DMSO as control for 18 h. Then the infected cells were subjected to immunostaining of RV VP6 antigens (red). Data are presented as mean ± SEM ( A, C, D, E ). The experiments were performed in triplicate ( A, C, D, E ), and the data are representative of two independent experiments ( A, C–G ). ns, not significant; ** P < 0.01, *** P < 0.001, **** P < 0.0001 (two-way ANOVA [ A, C, E ] and one-way ANOVA [ D ]).

Article Snippet: Human intestinal epithelial cell lines Caco-2 cells (ATCC: HTB-37) and HT-29 cells (ATCC: HTB-38) were from ATCC and kept in our institute.

Techniques: Expressing, Infection, Control, Virus, Immunostaining

NVP-HSP990 alters the life state of host cells. Caco-2 cells were mock-infected with PBS or infected with RV Wa or SA11 strains (MOI = 3) and further cultivated with DMEM containing 100 nM NVP-HSP990 or an equal volume of DMSO as a control for 24 h. Then, the infected cells were harvested for RNA-seq analysis. ( A ) Multiple differential scatter plots of compared groups. ( B ) Venn diagrams of up- and downregulated genes between compared groups. ( C, D ) Top 10 (ranked by descending Q value) upregulated ( C ) and downregulated ( D ) KEGG pathways in all mock-, Wa-, and SA11-infected Caco-2 cells. ( E, F ) Top 10 (ranked by descending Q value) upregulated ( E ) and downregulated ( F ) KEGG pathways in both Wa- and SA11-infected but not in mock-infected Caco-2 cells. *Q value <0.05.

Journal: Journal of Virology

Article Title: A small-molecule HSP90 inhibitor, NVP-HSP990, alleviates rotavirus infection

doi: 10.1128/jvi.01883-25

Figure Lengend Snippet: NVP-HSP990 alters the life state of host cells. Caco-2 cells were mock-infected with PBS or infected with RV Wa or SA11 strains (MOI = 3) and further cultivated with DMEM containing 100 nM NVP-HSP990 or an equal volume of DMSO as a control for 24 h. Then, the infected cells were harvested for RNA-seq analysis. ( A ) Multiple differential scatter plots of compared groups. ( B ) Venn diagrams of up- and downregulated genes between compared groups. ( C, D ) Top 10 (ranked by descending Q value) upregulated ( C ) and downregulated ( D ) KEGG pathways in all mock-, Wa-, and SA11-infected Caco-2 cells. ( E, F ) Top 10 (ranked by descending Q value) upregulated ( E ) and downregulated ( F ) KEGG pathways in both Wa- and SA11-infected but not in mock-infected Caco-2 cells. *Q value <0.05.

Article Snippet: Human intestinal epithelial cell lines Caco-2 cells (ATCC: HTB-37) and HT-29 cells (ATCC: HTB-38) were from ATCC and kept in our institute.

Techniques: Infection, Control, RNA Sequencing

NVP-HSP990 inhibited MAPK activation and facilitated expression of tight junction-associated proteins in intestinal cells. ( A ) MA104, Caco-2, and HT-29 cells were mock-infected with PBS or infected with RV Wa or SA11 strains (MOI = 3), followed by treatment of 100 nM HSP990 (+) or an equal volume of DMSO as a control (−) for 20 h. Then the infected cells were harvested for WB analysis of MAPK components. ( B ) Caco-2 cells were mock-infected with PBS, treated with 1 µM C16-PAF(C16), or infected with RV Wa or SA11 strains (MOI = 3), and then treated with 100 nM HSP990 (+) or DMSO as a control (−) for 20 h. The infected cells were harvested for WB analysis of MAPK components. ( C ) Caco-2 cells were mock-infected with PBS or infected with RV Wa or SA11 strains (MOI = 3), and then treated with 100 nM HSP990 (+) or DMSO as control (−) for 20 h. Then the infected cells were harvested for WB analysis of tight junction-associated proteins. Data are representative of three ( A ) and two ( B and C ) independent experiments.

Journal: Journal of Virology

Article Title: A small-molecule HSP90 inhibitor, NVP-HSP990, alleviates rotavirus infection

doi: 10.1128/jvi.01883-25

Figure Lengend Snippet: NVP-HSP990 inhibited MAPK activation and facilitated expression of tight junction-associated proteins in intestinal cells. ( A ) MA104, Caco-2, and HT-29 cells were mock-infected with PBS or infected with RV Wa or SA11 strains (MOI = 3), followed by treatment of 100 nM HSP990 (+) or an equal volume of DMSO as a control (−) for 20 h. Then the infected cells were harvested for WB analysis of MAPK components. ( B ) Caco-2 cells were mock-infected with PBS, treated with 1 µM C16-PAF(C16), or infected with RV Wa or SA11 strains (MOI = 3), and then treated with 100 nM HSP990 (+) or DMSO as a control (−) for 20 h. The infected cells were harvested for WB analysis of MAPK components. ( C ) Caco-2 cells were mock-infected with PBS or infected with RV Wa or SA11 strains (MOI = 3), and then treated with 100 nM HSP990 (+) or DMSO as control (−) for 20 h. Then the infected cells were harvested for WB analysis of tight junction-associated proteins. Data are representative of three ( A ) and two ( B and C ) independent experiments.

Article Snippet: Human intestinal epithelial cell lines Caco-2 cells (ATCC: HTB-37) and HT-29 cells (ATCC: HTB-38) were from ATCC and kept in our institute.

Techniques: Activation Assay, Expressing, Infection, Control

NVP-HSP990 mitigated disruption of tight junctions in RV infection. Caco-2 cells growing on coverslips were mock-infected or infected with RV Wa or SA11 strains (MOI = 3), and cultivated with DMEM containing 100 nM NVP-HSP990 or an equal volume of DMSO as a control after infection for another 18 h. Then the infected cells were applied for immunostaining for RV antigens (green), ZO-1 (red), and DAPI staining of nucleus (blue). Data are representative of two independent experiments.

Journal: Journal of Virology

Article Title: A small-molecule HSP90 inhibitor, NVP-HSP990, alleviates rotavirus infection

doi: 10.1128/jvi.01883-25

Figure Lengend Snippet: NVP-HSP990 mitigated disruption of tight junctions in RV infection. Caco-2 cells growing on coverslips were mock-infected or infected with RV Wa or SA11 strains (MOI = 3), and cultivated with DMEM containing 100 nM NVP-HSP990 or an equal volume of DMSO as a control after infection for another 18 h. Then the infected cells were applied for immunostaining for RV antigens (green), ZO-1 (red), and DAPI staining of nucleus (blue). Data are representative of two independent experiments.

Article Snippet: Human intestinal epithelial cell lines Caco-2 cells (ATCC: HTB-37) and HT-29 cells (ATCC: HTB-38) were from ATCC and kept in our institute.

Techniques: Disruption, Infection, Control, Immunostaining, Staining

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